Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Dec;49(12):5172-5.
doi: 10.1128/AAC.49.12.5172-5175.2005.

Cloning and functional analysis of the gene encoding the 33- to 36-kilodalton outer membrane protein associated with carbapenem resistance in Acinetobacter baumannii

Maria del Mar Tomás  1 , Alejandro Beceiro, Astrid Pérez, David Velasco, Rita Moure, Rosa Villanueva, Jesús Martínez-Beltrán, Germán Bou
Affiliations

Cloning and functional analysis of the gene encoding the 33- to 36-kilodalton outer membrane protein associated with carbapenem resistance in Acinetobacter baumannii

Maria del Mar Tomás et al. Antimicrob Agents Chemother. 2005 Dec.

Abstract

We investigated a multiresistant strain of Acinetobacter baumannii isolated in our hospital. Analysis of the N-terminal peptide sequence of the outer membrane proteins (OMPs) purified from the strain allowed us to clone and sequence the nucleotides of the gene encoding the 33- to 36-kDa OMP associated with carbapenem resistance in A. baumannii.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Electrophoretic analysis of OMPs (ca. 30 μg each) with a 10% sodium dodecyl sulfate-polyacrylamide gel with 6 M urea stained with Coomassie brilliant blue R-250. Lanes: Mw, molecular mass markers of the indicated sizes; JC10/01, purified OMPs from strain JC10/01; EG-1, purified OMPs from strain EG-1; EG-1rev, purified OMPs from strain EG-1rev. The arrow indicates the position of the 33- to 36-kDa OMP.
FIG. 2.
FIG. 2.
Electrophoretic analysis of OMPs (ca. 30 μg each) of JC10/01 strain transformed with the indicated plasmids with a 10% sodium dodecyl sulfate-polyacrylamide gel with 6 M urea stained with Coomassie brilliant blue R-250. Lanes: Mw, molecular mass markers of indicated sizes; JC10/01 pAT-RA-c, purified OMPs from strain JC10/01 as negative control; JC10/01 pAT-RA-34s, purified OMPs from strain JC10/01 expressing the 33- to 36-kDa OMP from JC7/04 isolate; JC10/01 pAT-RA-34r, purified OMPs from strain JC10/01 expressing the 33- to 36-kDa OMP from JC10/01 isolate. The arrow indicates the position of the 33- to 36-kDa OMP.
See this image and copyright information in PMC

References

    1. Afzal, M. S., and D. Livermore. 1998. Worldwide emergence of carbapenem-resistant Acinetobacter spp. J. Antimicrob. Chemother. 41:576-577. - PubMed
    1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402. - PMC - PubMed
    1. Barbe, V., D. Vallenet, N. Fonknechten, and A. Kreimeyer. 2004. Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium. Nucleic Acids Res. 32:5766-5779. - PMC - PubMed
    1. Baumann, P. 1968. Isolation of Acinetobacter from soil and water. J. Bacteriol. 96:39-42. - PMC - PubMed
    1. Bergogne-Bérézin, E., and K. J. Towner. 1996. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin. Microbiol. Rev. 9:148-165. - PMC - PubMed

Publication types

MeSH terms

Substances

Associated data