Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Jul 15;89(14):6232-6.
doi: 10.1073/pnas.89.14.6232.

Targeted oncogene activation by site-specific recombination in transgenic mice

M Lakso  1 , B Sauer, B Mosinger Jr, E J Lee, R W Manning, S H Yu, K L Mulder, H Westphal
Affiliations

Targeted oncogene activation by site-specific recombination in transgenic mice

M Lakso et al. Proc Natl Acad Sci U S A. .

Abstract

An efficient and accurate method for controlled in vivo transgene modulation by site-directed recombination is described. Seven transgenic mouse founder lines were produced carrying the murine lens-specific alpha A-crystallin promoter and the simian virus 40 large tumor-antigen gene sequence, separated by a 1.3-kilobase-pair Stop sequence that contains elements preventing expression of the large tumor-antigen gene and Cre recombinase recognition sites. Progeny from two of these lines were mated with transgenic mice expressing the Cre recombinase under control of either the murine alpha A-crystallin promoter or the human cytomegalovirus promoter. All double-transgenic offspring developed lens tumors. Subsequent analysis confirmed that tumor formation resulted from large tumor-antigen activation via site-specific, Cre-mediated deletion of Stop sequences.

PubMed Disclaimer

References

    1. New Biol. 1989 Nov;1(2):193-204 - PubMed
    1. Genome. 1989;31(2):520-7 - PubMed
    1. Science. 1991 Mar 15;251(4999):1351-5 - PubMed
    1. Crit Rev Biochem Mol Biol. 1990;25(6):415-56 - PubMed
    1. Cell. 1989 Nov 3;59(3):499-509 - PubMed