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. 2005 Nov;11(11):1701-7.
doi: 10.3201/eid1111.050851.

Toscana virus in Spain

Sara Sanbonmatsu-Gámez  1 , Mercedes Pérez-Ruiz, Ximena Collao, María Paz Sánchez-Seco, Francisco Morillas-Márquez, Manuel de la Rosa-Fraile, José Maria Navarro-Mari, Antonio Tenorio
Affiliations

Toscana virus in Spain

Sara Sanbonmatsu-Gámez et al. Emerg Infect Dis. 2005 Nov.

Abstract

Toscana virus (TOSV, Phlebovirus, family Bunyaviridae) infection is one of the most prevalent arboviruses in Spain. Within the objectives of a multidisciplinary network, a study on the epidemiology of TOSV was conducted in Granada, in southern Spain. The overall seroprevalence rate was 24.9%, significantly increasing with age. TOSV was detected in 3 of 103 sandfly pools by viral culture or reverse transcription-polymerase chain reaction from a region of the L gene. Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3. Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain. These findings demonstrate the circulation of at least 2 different lineages of TOSV in the Mediterranean basin, the Italian lineage and the Spanish lineage.

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Figures

Figure 1
Figure 1
Upper map, geographic situation of the study population (Granada province) in Spain; lower, distribution of geographic areas in Granada province for the seroprevalence study of anti–Toscana virus immunoglobulin G antibodies.
Figure 2
Figure 2
Phylogenetic trees illustrating the relationship between representatives of different phleboviruses and the Spanish Toscana virus (TOSV) within the nucleotide (nt, L) and the deduced amino acid sequences (aa, L) of the L (partial) gene. GR40 and GR41 correspond to TOSV isolates obtained from sand flies. GR79 corresponds to a reverse transcription–polymerase chain reaction–positive pool of sandflies. STI1–STI11 were recovered from patients with aseptic meningitis diagnosed from 1988 to 2002 as described in (17). Abbreviations and GenBank accession numbers are indicated in the text. Bootstrapping values >50 are indicated at the nodes.
Figure 3
Figure 3
Phylogenetic trees illustrating the relationship between representatives of different phleboviruses and the Spanish Toscana virus isolates within the nucleotide (nt, N) and deduced amino acids sequences (aa, N) of the N gene.
See this image and copyright information in PMC

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