doi: 10.1016/0165-2427(92)90057-w.
Determination of optimal in vitro conditions for caprine alternative complement pathway assay
Affiliations
- PMID: 1632071
- DOI: 10.1016/0165-2427(92)90057-w
Item in Clipboard
Determination of optimal in vitro conditions for caprine alternative complement pathway assay
Vet Immunol Immunopathol.
1992 May.
Abstract
Optimal in vitro testing conditions for caprine alternative complement pathway assay were determined. Effects of the following variables were tested: heterologous erythrocytes; pH, ionic strength and Mg2+ ion concentration of the complement diluent; incubation time and temperature. Rabbit erythrocytes were the optimal target cells. The optimal buffer conditions were: pH 8.0, ionic strength 0.06 mmol NaCl and 5mmol Mg2+ ion. Optimal incubation time and temperature were 75 min and 30 degrees C, respectively.
Similar articles
-
Studies on the haemolytic complement of the dromedary camel (Camelus dromedarius). I. Classical pathway haemolytic activity in serum.Vet Immunol Immunopathol. 1995 Jun;46(3-4):337-47. doi: 10.1016/0165-2427(94)05367-2. Vet Immunol Immunopathol. 1995. PMID: 7502492
-
Study of the optimal reaction conditions for assay of the mouse alternative complement pathway.J Immunol Methods. 1985 Dec 27;85(2):233-43. doi: 10.1016/0022-1759(85)90134-6. J Immunol Methods. 1985. PMID: 4078314
-
A study of optimal reaction conditions for an assay of the human alternative complement pathway.Am J Clin Pathol. 1983 Jan;79(1):65-72. doi: 10.1093/ajcp/79.1.65. Am J Clin Pathol. 1983. PMID: 6849295
-
Hemolytic assay for goat (caprine) and swine (porcine) complement.Vet Immunol Immunopathol. 1980 Dec;1(4):317-28. doi: 10.1016/0165-2427(80)90011-2. Vet Immunol Immunopathol. 1980. PMID: 15615050
-
Erythrocyte-bound complement components in health and disease.Prog Clin Biol Res. 1980;43:133-55. Prog Clin Biol Res. 1980. PMID: 6999500 Review. No abstract available.
Cited by
-
The complement system of the goat: haemolytic assays and isolation of major proteins.BMC Vet Res. 2012 Jun 26;8:91. doi: 10.1186/1746-6148-8-91. BMC Vet Res. 2012. PMID: 22734447 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
