doi: 10.1021/ol052125k.
On-bead fluorescence assay for serine/threonine kinases
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- PMID: 16320992
- DOI: 10.1021/ol052125k
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On-bead fluorescence assay for serine/threonine kinases
Org Lett.
.
Abstract
[chemical reaction: see text]. A novel fluorescence-based assay for serine/threonine kinases is described. Base-mediated beta-elimination of the phosphate moiety and the Michael addition of a thiol-containing fluorescent molecule allows convenient and efficient detection of the enzyme activity. This approach may be broadly applicable to various serine/threonine kinases.
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