Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Dec 8;7(25):5565-8.
doi: 10.1021/ol052125k.

On-bead fluorescence assay for serine/threonine kinases

Shoji Akita  1 , Naoki Umezawa, Tsunehiko Higuchi
Affiliations

On-bead fluorescence assay for serine/threonine kinases

Shoji Akita et al. Org Lett. .

Abstract

[chemical reaction: see text]. A novel fluorescence-based assay for serine/threonine kinases is described. Base-mediated beta-elimination of the phosphate moiety and the Michael addition of a thiol-containing fluorescent molecule allows convenient and efficient detection of the enzyme activity. This approach may be broadly applicable to various serine/threonine kinases.

PubMed Disclaimer

Publication types

LinkOut - more resources