Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Dec;14(12):3140-4.
doi: 10.1110/ps.051848105.

2005 Irving Sigal Young Investigator Award

Tom W Muir  1
Affiliations

2005 Irving Sigal Young Investigator Award

Tom W Muir. Protein Sci. 2005 Dec.
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Principle of EPL in which synthetic and recombinant proteins are chemically ligated together using the NCL reaction. SPPS, solid phase peptide synthesis.
Figure 2.
Figure 2.
Examples of biochemical and biophysical probes incorporated into proteins using EPL. The approach has also been used to ligate proteins to DNA and surfaces and to circularize recombinant proteins.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Chong, S., Mersha, F.B., Comb, D.G., Scott, M.E., Landry, D., Vence, L.M., Perler, F.B., Benner, J., Kucera, R.B., Hirvonen, C.A., et al. 1997. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192 271–281. - PubMed
    1. Dawson, P.E. and Kent, S.B.H. 2000. Synthesis of native proteins by chemical ligation. Annu. Rev. Biochem. 69 923–960. - PubMed
    1. Dawson, P.E., Muir, T.W., Clark-Lewis, I., and Kent, S.B.H. 1994. Synthesis of proteins by native chemical ligation. Science 266 776–779. - PubMed
    1. Erlandson, D.A., Chytil, M., and Verdine, G.L. 1996. The leucine zipper domain controls the orientation of AP-1 in the NFAT·AP-1·DNA complex. Chem. Biol. 3 981–991. - PubMed
    1. Gaertner, H.F., Rose, K., Cotton, R., Timms, D., Camble, R., and Offord, R.E. 1992. Construction of protein analogues by site-specific condensation of unprotected peptides. Bioconjugate Chem. 3 262–268. - PubMed

Personal name as subject

LinkOut - more resources