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Comparative Study
. 1975 Feb;85(2):312-7.

A radioisotopic assay for proline oxidase activity

J M Phang, S J Downing, D L Valle, E M Kowaloff
  • PMID: 163285
Comparative Study

A radioisotopic assay for proline oxidase activity

J M Phang et al. J Lab Clin Med. 1975 Feb.

Abstract

We developed a radioisotopic assay for proline oxidase in which product deltal-pyrroline-5-carboxylate-14-C is reacted with o-aminobenzaldehyde and the radioactivity trapped as the dihydroquinazolinium compound is recovered by ion-exchange chromatography. The sensitivity of this method allows the measurement of proline oxidase activity in small specimens (10 to 20 mg.) of liver.

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