Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Jun;63(11):1870-8.
doi: 10.1016/j.chemosphere.2005.10.022. Epub 2005 Dec 5.

Analysis of estrogenic activity in coastal surface waters of the Baltic Sea using the yeast estrogen screen

Affiliations

Analysis of estrogenic activity in coastal surface waters of the Baltic Sea using the yeast estrogen screen

Iris-Constanze Beck et al. Chemosphere. 2006 Jun.

Abstract

In the present study, the yeast estrogen screen (YES) has been used to assess the estrogenic activity in surface waters of a coastal region in the German Baltic Sea. Solid-phase extraction using the copolymer Oasis HLB followed by a clean-up on silica was carried out on approximately 50-l water samples. From the final 400 microl extract volume, 100 microl aliquots were used for the measurement of estrogenic activity and for chemical analysis, which was performed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). From 29 samples taken during two campaigns (2003 and 2004) at five different stations 27 samples showed an estrogenic response higher than 10%. The response in the YES was expressed as measured estradiol equivalents (EEQs), which were in the range of 0.01 (Darss Peninsula) to 0.82 ng/l (Inner Wismar Bay). Samples from stations located in inner coastal waters showed higher estrogenic activities than those from outer located stations. A comparison of measured estrogenicity (YES) and calculated estrogenicity (chemical analysis) showed significant differences, probably due to the presence of anti-estrogenic compounds and/or the estrogenic activity of unknown, not identified contaminants. The main contributors to the overall estrogenic activity were synthetic and natural hormones.

PubMed Disclaimer

LinkOut - more resources