Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Dec;43(12):5912-5.
doi: 10.1128/JCM.43.12.5912-5915.2005.

Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species

Oliver Goldenberg  1 , Stefanie Herrmann, Thomas Adam, Gina Marjoram, George Hong, Ulf B Göbel, Barbara Graf
Affiliations

Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species

Oliver Goldenberg et al. J Clin Microbiol. 2005 Dec.

Abstract

A novel denaturing high-performance liquid chromatography (DHPLC)-based technique allows rapid high-resolution analysis of PCR products. We used this technique for unequivocal molecular identification of seven Candida species. We show the application of this PCR/DHPLC approach for direct detection and identification of yeast species from blood cultures and for detection of Candida colonization in the gastrointestinal tract of allogeneic transplant patients.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
DHPLC peak profile of mixed fungal amplicons. Shown are mixtures of PCR-amplified ITS2 DNA fragments from C. membranaefaciens (peak 1), C. tropicalis (peak 2), C. parapsilosis (peak 3), C. magnoliae (peak 4), C. lusitaniae (peak 5), C. dubliniensis (peak 6), C. albicans (peak 7), C. inconspicua (peak 8), C. krusei (peak 9), C. neoformans (peak 10), C. kefyr (peak 11), and C. glabrata (peak 12).
FIG. 2.
FIG. 2.
Peak profiles obtained from Candida variants. Profiles consisting of only one major peak were designated type I. Dashed line, marker profile containing type I reference peaks and the type II variant of C. tropicalis. (A) Besides type I, six additional Candida albicans variants (types II to VII) were identified by analyzing clinical isolates and blood culture samples. (B) Candida glabrata variants (types I to VI). (C) Three variants were observed for Candida tropicalis (types I to III). Note that the marker profile contains types I and II of C. tropicalis.
See this image and copyright information in PMC

References

    1. Chen, Y. C., S. C. Chang, C. C. Sun, L. S. Yang, W. C. Hsieh, and K. T. Luh. 1997. Secular trends in the epidemiology of nosocomial fungal infections at a teaching hospital in Taiwan, 1981 to 1993. Infect. Control Hosp. Epidemiol. 18:369-375. - PubMed
    1. Chen, Y. C., J. D. Eisner, M. M. Kattar, S. L. Rassoulian-Barrett, K. LaFe, U. Bui, A. P. Limaye, and B. T. Cookson. 2001. Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts. J. Clin. Microbiol. 39:4042-4051. - PMC - PubMed
    1. De Baere, T., G. Claeys, D. Swinne, G. Verschraegen, A. Muylaert, C. Massonet, and M. Vaneechoutte. 2002. Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2). BMC Microbiol. 2:21. - PMC - PubMed
    1. Domann, E., G. Hong, C. Imirzalioglu, S. Turschner, J. Kuhle, C. Watzel, T. Hain, H. Hossain, and T. Chakraborty. 2003. Culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. J. Clin. Microbiol. 41:5500-5510. - PMC - PubMed
    1. Frueh, F. W., and M. Noyer-Weidner. 2003. The use of denaturing high-performance liquid chromatography (DHPLC) for the analysis of genetic variations: impact for diagnostics and pharmacogenetics. Clin. Chem. Lab. Med. 41:452-461. - PubMed

Publication types

MeSH terms

LinkOut - more resources