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. 2005 Dec;43(12):6194-6.
doi: 10.1128/JCM.43.12.6194-6196.2005.

PCR protocol for specific identification of Candida nivariensis, a recently described pathogenic yeast

Julia Alcoba-Flórez  1 , María del Pilar Arévalo, F Javier González-Paredes, Josep Cano, Josep Guarro, Eduardo Pérez-Roth, Sebastián Méndez-Alvarez
Affiliations

PCR protocol for specific identification of Candida nivariensis, a recently described pathogenic yeast

Julia Alcoba-Flórez et al. J Clin Microbiol. 2005 Dec.

Abstract

Candida nivariensis is a recently described pathogenic yeast closely related to Candida glabrata. We developed a specific set of oligonucleotide primers based on the internal transcribed spacer regions of the rRNA gene for the rapid identification of C. nivariensis. PCR with these primers amplified a 206-bp amplicon from C. nivariensis.

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Figures

FIG. 1.
FIG. 1.
Agarose gel electrophoresis of multiplex PCR amplification products D1/D2 (NL) and ITS1 (NIV). Lanes: M, 100-bp DNA ladder (Roche Diagnostics, Mannheim, Germany); 1, C. albicans ATCC 90028; 2, C. glabrata ATCC 90030; 3, C. krusei ATCC 6258; 4, C. parasilopsis ATCC 22019; 5, C. lusitaniae; 6, C. dubliniensis; 7, C. tropicalis; 8 to 10, C. nivariensis; 11, control without a DNA template.
See this image and copyright information in PMC

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