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. 2006 Feb;30(1):43-7.
doi: 10.1007/s00264-005-0023-5. Epub 2005 Dec 7.

Changes in biochemical parameters after anterior cruciate ligament injury

H Higuchi  1 , K Shirakura, M Kimura, M Terauchi, T Shinozaki, H Watanabe, K Takagishi
Affiliations

Changes in biochemical parameters after anterior cruciate ligament injury

H Higuchi et al. Int Orthop. 2006 Feb.

Abstract
in English, French

We studied the biochemical characteristics of human knees with deficient anterior cruciate ligaments (ACL) and analysed their relationship to the time after ligamentous injury. Thirty-two patients with isolated ACL-injured knees and six healthy volunteers were enrolled. Synovial fluid samples were centrifuged after aspiration during arthroscopic examination, and aliquots of supernatant were frozen and stored at -80 degrees C. The samples were analysed for interleukin (IL)-1beta, tumour necrosis factor (TNF)-alpha, IL-6, matrix metalloproteinase (MMP)-3, and tissue inhibitor of metalloproteinase (TIMP)-1 using commercially available sandwich enzyme-linked immunosorbent assay. In fluid from ACL-injured knees, the average concentrations of IL-6, MMP-3 and TIMP-1 were highly elevated in comparison with normal controls. There was a statistically significant correlation between the concentrations of MMP-3 and IL-6. The IL-6 and TIMP-1 concentrations were interrelated. The concentration of MMP-3 remained high, independent of the duration since the injury, whereas the TIMP-1 and IL-6 levels decreased. The results suggest that the timing of the treatment of an ACL-injured knee might be of importance.

Nous avons étudié les caractéristiques biochimiques des genoux humains avec un ligament croisé antérieur défectueux (LCA) et avons analysé leur rapport au temps après la lésion ligamentaire. Trente-deux malades avec une lésion isolée du LCA et six volontaires sains ont été enrôlés. Des échantillons du liquide synovial ont été centrifugés après aspiration pendant un examen arthroscopique, et le surnageant a été congelé et entreposé à −80 degrés Celsius. Les échantillons ont été analysés pour l'interleukine (IL)-1β, le facteur de nécrose tumorale (TNF), IL 6, la métalloprotéinase de la matrice (MMP)-3 et l'inhibiteur tissulaire de la métalloproteinase (TIMP)-1. Dans le liquide des genoux traumatisés les concentrations moyennes de IL 6, MMP-3 et TIMP-1 étaient très élevées en comparaison des contrôles normaux. Il y avait une corrélation statistiquement significative entre les concentrations de MMP-3 et IL 6. Les concentrations de IL 6 et TIMP-1 ont été mises en corrélation. La concentration de MMP-3 est restée élevée, indépendante du délai de la lésion ligamentaire, alors que le niveau de TIMP-1 et IL6 a diminué. Les résultats suggèrent que le délai d'intervention sur un genou ayant une rupture du croisé antérieur peut être un élément d'importance.

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Figures

Fig 1
Fig 1
Relation between synovial fluid concentrations of MMP-3 and TIMP-1 in the ACL-deficient knees. There was a positive correlation between them (P<0.01)
Fig 2
Fig 2
Relation between synovial fluid concentrations of IL-6 and TIMP-1 in ACL-deficient knees. There was a significant correlation between them (P<0.01)
Fig 3
Fig 3
Synovial fluid concentrations of IL-6 in ACL-injured knees with time from injury. The IL-6 concentration was high in many knees within 50 weeks after ACL injury
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