Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Jan;15(1):167-72.
doi: 10.1128/JVI.15.1.167-172.1975.

Electron microscope localization of a protein bound near the origin of simian virus 40 DNA replication

J Griffith, M Dieckmann, P Berg

Electron microscope localization of a protein bound near the origin of simian virus 40 DNA replication

J Griffith et al. J Virol. 1975 Jan.

Abstract

A salt-stable complex of protein and viral DNA obtained from Simian virus 40 (SV40)-infected monkey cells or mature SV40 virions has a novel structure. When viewed by high resolution electron microscopy, the circular SV40 DNA molecule has bound to it one to three globular protein "knobs". Using ecoRI and hpaII restriction endonucleases, each of which can cleave SV40 DNA once at a known location (10, 11, 12, 14), the bound protein can be localized at 0.7 plus or minis 0.05 on the SV40 DNA physical map (SV40 fractional length, clockwise from the ecoRI endonuclease-cleavage site).

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Virol. 1972 Jun;9(6):923-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1971 May;68(5):1032-6 - PubMed
    1. J Virol. 1971 Oct;8(4):363-71 - PubMed
    1. J Virol. 1972 Feb;9(2):317-25 - PubMed
    1. J Virol. 1972 Jun;9(6):956-68 - PubMed

Publication types

LinkOut - more resources