Comparison of Nitrogen Fixation Activity in Tall and Short Spartina alterniflora Salt Marsh Soils

Abstract

A comparison of the N(2) fixers in the tall Spartina alterniflora and short S. alterniflora marsh soils was investigated. Zero-order kinetics and first-order kinetics of acetylene reduction were used to describe the activity of the N(2) fixers in marsh soil slurries. It was found that the V(max) values were approximately 10 times greater for the N(2) fixers in the tall Spartina than in the short Spartina marsh when raffinose was used as the energy source. In addition, the (K(s) + S(n)) values were approximately 4 to 15 times lower for the N(2) fixers in the tall Spartina than in short Spartina marsh. First-order kinetics of nitrogen fixation for several substrates indicate that the N(2) fixers in the tall Spartina marsh were two to seven times more active than those in the short Spartina marsh. Ammonium chloride (25 mug/ml) did not inhibit nitrogen fixation in the tall Spartina marsh, but there was a 50% inhibition in nitrogen fixation in the short Spartina marsh. On the other hand, sodium nitrate inhibited nitrogen fixation almost 100% at 25 mug/ml in both soil environments. Amino nitrogen (25 to 100 mug/ml) had little or no effect on nitrogen fixation. The results indicate that the N(2) fixers in the tall Spartina marsh were physiologically more responsive to nutrient addition than those in the short Spartina marsh. This difference in the two populations may be related to the difference in daily tidal influence in the respective areas and thus provide another explanation for the enhanced S. alterniflora production in the creek bank soil system.