Production of NO(2) and N(2)O by Nitrifying Bacteria at Reduced Concentrations of Oxygen

Abstract

Pure cultures of the marine ammonium-oxidizing bacterium Nitrosomonas sp. were grown in the laboratory at oxygen partial pressures between 0.005 and 0.2 atm (0.18 to 7 mg/liter). Low oxygen conditions induced a marked decrease in the rate for production of NO(2), from 3.6 x 10 to 0.5 x 10 mmol of NO(2) per cell per day. In contrast, evolution of N(2)O increased from 1 x 10 to 4.3 x 10 mmol of N per cell per day. The yield of N(2)O relative to NO(2) increased from 0.3% to nearly 10% (moles of N in N(2)O per mole of NO(2)) as the oxygen level was reduced, although bacterial growth rates changed by less than 30%. Nitrifying bacteria from the genera Nitrosomonas, Nitrosolobus, Nitrosospira, and Nitrosococcus exhibited similar yields of N(2)O at atmospheric oxygen levels. Nitrite-oxidizing bacteria (Nitrobacter sp.) and the dinoflagellate Exuviaella sp. did not produce detectable quantities of N(2)O during growth. The results support the view that nitrification is an important source of N(2)O in the environment.