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. 1981 Apr;41(4):1043-5.
doi: 10.1128/aem.41.4.1043-1045.1981.

Detection of rotavirus in sewage

J Steinmann  1
Affiliations

Detection of rotavirus in sewage

J Steinmann. Appl Environ Microbiol. 1981 Apr.

Abstract

FOR DETECTION OF ROTAVIRUS, DOMESTIC SEWAGE WAS CONCENTRATED BY TWO DIFFERENT METHODS: (i) adsorption to and elution from positively charged Seitz filters, followed by ultracentrifugation, and (ii) chemical precipitation. The concentrated fluids were tested by an enzyme-linked immunosorbent assay and electron microscopy. In 6 of 24 (25%) samples, rotavirus was detectable after the combined filtration and ultracentrifugation technique with both an enzyme-linked immunosorbent assay and electron microscopy. No positive results were obtained after chemical precipitation.

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