Transposon mutagenesis in rhizobia which can nodulate both legumes and the nonlegume parasponia
- PMID: 16345924
- PMCID: PMC241805
- DOI: 10.1128/aem.43.1.233-236.1982
Transposon mutagenesis in rhizobia which can nodulate both legumes and the nonlegume parasponia
Abstract
The successful use of transposon mutagenesis in a slow-growing Rhizobium permitted the isolation of auxotrophic and symbiotically defective mutants. In these mutants, it was shown that different single-copy Tn5 insertions had occurred. Mutant phenotypes indicated that the control of nitrogenase activity in vitro and in planta is different.
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