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Comparative Study
. 1992 Jun;37(3):337-43.
doi: 10.1016/0166-0934(92)90033-a.

Rapid diagnosis of influenza A. Comparison with ELISA immunocapture and culture

J J Chomel  1 , M F Remilleux, P Marchand, M Aymard
Affiliations
Comparative Study

Rapid diagnosis of influenza A. Comparison with ELISA immunocapture and culture

J J Chomel et al. J Virol Methods. 1992 Jun.

Abstract

The Directigen Flu-A is an enzyme immunoassay for detecting in 15 min the influenza A nucleoproteinic antigen directly from specimens after passive adsorption on a cellulose membrane. The test was assessed using 160 frozen (-20 degrees C) specimens collected during the 1988-1989 A/H1N1 influenza epidemic and the 1989-1990 A/H3N2 epidemic. Compared to the ELISA immunocapture test, the sensitivity of the commercial test was 87.8% and the specificity was 97.6%. When compared to isolation of viruses on LLCMK2 cells and/or chicken embryo, the sensitivity was 84%. No cross-reaction was found with other respiratory disease viruses. The feasibility, practicability and rapidity of the test make it a test of choice for rapid diagnosis of influenza A.

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