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. 1982 May;43(5):1208-9.
doi: 10.1128/aem.43.5.1208-1209.1982.

Purification of Poly-beta-Hydroxybutyrate by Density Gradient Centrifugation in Sodium Bromide

K W Nickerson  1
Affiliations

Purification of Poly-beta-Hydroxybutyrate by Density Gradient Centrifugation in Sodium Bromide

K W Nickerson. Appl Environ Microbiol. 1982 May.

Abstract

Fractionation of fully sporulated cultures of Bacillus thuringiensis by density gradient centrifugation in NaBr produced two bands which were identified as poly-beta-hydroxybutyrate. This technique generated high yields of membrane-bound and unbound granules of exceptional purity and degree of polymerization.

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References

    1. Adv Microb Physiol. 1973;10:135-266 - PubMed
    1. Biochemistry. 1968 Oct;7(10):3676-81 - PubMed
    1. J Bacteriol. 1961 Jul;82:33-6 - PubMed
    1. Appl Environ Microbiol. 1978 Oct;36(4):625-6 - PubMed
    1. Appl Microbiol. 1974 Jul;28(1):129-32 - PubMed

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