Transformation of Streptococcus lactis Protoplasts by Plasmid DNA
- PMID: 16346019
- PMCID: PMC244210
- DOI: 10.1128/aem.43.5.1213-1215.1982
Transformation of Streptococcus lactis Protoplasts by Plasmid DNA
Abstract
Polyethylene glycol-treated protoplasts prepared from Streptococcus lactis LM3302, a lactose-negative (Lac) derivative of S. lactis ML3, were transformed to lactose-fermenting ability by a transductionally shortened plasmid (pLM2103) coding for lactose utilization.
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