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. 1983 Feb;45(2):701-2.
doi: 10.1128/aem.45.2.701-702.1983.

Method for rapid detection of cyanogenic bacteria

K F Castric  1 , P A Castric
Affiliations

Method for rapid detection of cyanogenic bacteria

K F Castric et al. Appl Environ Microbiol. 1983 Feb.

Abstract

An agar plate method is described in which the production of hydrogen cyanide by as many as 50 microbial isolates per plate may be detected. Cyanide produced by the organisms reacts with copper(II) ethylacetoacetate and 4,4'-methylenebis-(N,N-dimethylaniline) in a paper disk suspended above the microbial colonies. Cell growth occurs in depressions in the agar surface, which allows separation of colonies and enhances sensitivity of hydrogen cyanide detection.

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References

    1. Can J Microbiol. 1975 May;21(5):613-18 - PubMed
    1. Analyst. 1966 Apr;91(81):282-4 - PubMed

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