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. 1983 Jun;45(6):1956-9.
doi: 10.1128/aem.45.6.1956-1959.1983.

Simple Assay for Accurate Determination of [S]sulfate Reduction Activity

H R Rosser  1 , W A Hamilton
Affiliations

Simple Assay for Accurate Determination of [S]sulfate Reduction Activity

H R Rosser et al. Appl Environ Microbiol. 1983 Jun.

Abstract

A method is described for the assay of [S]sulfate reduction in which filter paper wicks are used to trap [S]sulfide. The simplicity of the technique enables large numbers of samples to be conveniently processed. Enhanced sensitivity is achieved since all acid-volatile [S]sulfides produced during the incubation period are counted. Recovery of radioactivity from added Na(2)S is excellent (mean, 100.1%; standard deviation, 1.81; n = 9) and is unaffected by sulfide concentrations of up to 400 mug per sample. Field trial results with anoxic sediment samples are presented.

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References

    1. Appl Environ Microbiol. 1978 Aug;36(2):297-305 - PubMed
    1. Appl Environ Microbiol. 1981 Mar;41(3):822-5 - PubMed
    1. Can J Microbiol. 1974 Jun;20(6):825-31 - PubMed

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