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. 1983 Nov;46(5):1080-3.
doi: 10.1128/aem.46.5.1080-1083.1983.

Confirmation of Occurrence of Hydroxamate Siderophores in Soil by a Novel Escherichia coli Bioassay

P E Powell  1 , P J Szaniszlo, C P Reid
Affiliations

Confirmation of Occurrence of Hydroxamate Siderophores in Soil by a Novel Escherichia coli Bioassay

P E Powell et al. Appl Environ Microbiol. 1983 Nov.

Abstract

The occurrence of ferrichrome-type hydroxamate siderophores in soil was confirmed. In the presence of the iron-scavenging chelator ethylenediamine[di(o-hydroxyphenylacetic)acid], soil extract stimulated the growth of an Escherichia coli strain possessing the ferrichrome transport protein (TonA) but did not stimulate growth of a strain lacking this protein (TonA). The siderophore concentration in a 1:1 (soil-water) extract was estimated to be approximately 78 nM. Specificity of the assay was supported by the absence of significant differential strain responses to ferric citrate, ferric 2,3-dihydroxybenzoate, enterochelin, ferrioxamine B, coprogen, and triacetylfusigen.

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References

    1. Biochemistry. 1971 Apr 13;10(8):1483-8 - PubMed
    1. Experientia. 1964 Aug 15;20(8):430-1 - PubMed
    1. J Bacteriol. 1980 Jul;143(1):256-64 - PubMed
    1. Infect Immun. 1981 Aug;33(2):540-5 - PubMed
    1. J Bacteriol. 1975 Nov;124(2):704-12 - PubMed

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