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. 1985 Sep;50(3):693-5.
doi: 10.1128/aem.50.3.693-695.1985.

Genetic Recombination and Transformation in Protoplasts of Thermomonospora fusca

K A Pidcock  1 , B S Montenecourt, J A Sands
Affiliations

Genetic Recombination and Transformation in Protoplasts of Thermomonospora fusca

K A Pidcock et al. Appl Environ Microbiol. 1985 Sep.

Abstract

Protoplasts were produced from the thermophilic actinomycete Thermomonospora fusca and were regenerated to 0.1% of the direct count on regeneration agar. Recombination after protoplast fusion was demonstrated with drug-resistant mutants of T. fusca YX. A single thiostrepton-resistant colony was isolated after transformation of T. fusca YX with the streptomycete vector pIJ702, providing the first evidence for transformation in the genus Thermomonospora and suggesting that some mesophilic streptomycete genes can be expressed in thermophilic actinomycetes. Of 20 thermophilic actinomycete strains isolated from self-heated composts, 3 were found to harbor native plasmid DNA, providing potential sequences for the development of Thermomonospora-Streptomyces shuttle vectors.

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References

    1. Nucleic Acids Res. 1978 Apr;5(4):1139-52 - PubMed
    1. Nature. 1978 Apr 27;272(5656):792-5 - PubMed
    1. J Gen Microbiol. 1974 Feb;80(2):389-400 - PubMed
    1. Soc Appl Bacteriol Symp Ser. 1973 Jan;2:231-51 - PubMed
    1. Nature. 1980 Jul 31;286(5772):525-7 - PubMed

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