Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Feb;51(2):238-43.
doi: 10.1128/aem.51.2.238-243.1986.

Solid Medium for Culturing Black Smoker Bacteria at Temperatures to 120 degrees C

J W Deming  1 , J A Baross
Affiliations

Solid Medium for Culturing Black Smoker Bacteria at Temperatures to 120 degrees C

J W Deming et al. Appl Environ Microbiol. 1986 Feb.

Abstract

A solid, highly thermostable medium, based on the new gelling agent GELRITE, was devised to facilitate the culturing of extremely thermophilic microorganisms from submarine hydrothermal vents. The medium remained solid at temperatures to 120 degrees C at vapor pressures and hydrostatic pressures to 265 atm. It proved useful to its maximum tested limits in isolating colonies of black smoker bacteria from hydrothermal fluids recently collected at the Juan de Fuca Ridge in the Pacific Ocean.

PubMed Disclaimer

References

    1. Appl Environ Microbiol. 1978 Dec;36(6):966-8 - PubMed
    1. Adv Microb Physiol. 1979;19:149-243 - PubMed
    1. Appl Environ Microbiol. 1977 May;33(5):1225-8 - PubMed
    1. Appl Environ Microbiol. 1982 May;43(5):1086-91 - PubMed
    1. Appl Environ Microbiol. 1984 Feb;47(2):427-9 - PubMed