Isolation and Characterization of Coproporphyrin Produced by Four Subspecies of Bacillus thuringiensis
- PMID: 16347008
- PMCID: PMC238905
- DOI: 10.1128/aem.51.3.481-486.1986
Isolation and Characterization of Coproporphyrin Produced by Four Subspecies of Bacillus thuringiensis
Abstract
It was found by using spectrophotometric, spectrofluorometric, and high-pressure liquid chromatography that four subspecies of Bacillus thuringiensis produce coproporphyrin. The porphyrin isomer was identified as coproporphyrin I for B. thuringiensis subsp. kurstaki (HD1). The porphyrin was isolated both from spores and from a variety of spent growth media. The quantity of porphyrin released by each Bacillus subspecies differed. The rank order of porphyrin production follows: B. thuringiensis subsp. kurstaki HD1 > B. thuringiensis subsp. thuringiensis HD27 > B. thuringiensis subsp. thuringiensis HD41 > B. thuringiensis subsp. darmstadiensis HD199.
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