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. 1987 May;53(5):1034-41.
doi: 10.1128/aem.53.5.1034-1041.1987.

Isolation of a Cellodextrinase from Bacteroides succinogenes

L Huang  1 , C W Forsberg
Affiliations

Isolation of a Cellodextrinase from Bacteroides succinogenes

L Huang et al. Appl Environ Microbiol. 1987 May.

Abstract

An enzyme which released the cellobiose group from p-nitrophenyl cellobioside was isolated from the periplasmic space of Bacteroides succinogenes grown on Avicel crystalline cellulose in a continuous cultivation system and separated from endoglucanases by column chromatography. The molecular weight of the enzyme was approximately 40,000, as estimated by gel filtration. The enzyme has an isoelectric point of 4.9. The enzyme exhibited low hydrolytic activity on acid-swollen cellulose and practically no activity on carboxymethyl cellulose, Avicel cellulose, and cellobiose, but it hydrolyzed p-nitrophenyl lactoside and released cellobiose from cellotriose and from higher cello-oligosaccharides. These data demonstrate that the enzyme is a cellodextrinase with an exotype of function.

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References

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