Production of ethane, ethylene, and acetylene from halogenated hydrocarbons by methanogenic bacteria

Abstract

Several methanogenic bacteria were shown to produce ethane, ethylene, and acetylene when exposed to the halogenated hydrocarbons bromoethane, dibromo- or dichloroethane, and 1,2-dibromoethylene, respectively. They also produced ethylene when exposed to the coenzyme M analog and specific methanogenic inhibitor bromoethanesulfonic acid. The production of these gases from halogenated hydrocarbons has a variety of implications concerning microbial ecology, agriculture, and toxic waste treatment. All halogenated aliphatic compounds tested were inhibitory to methanogens. Methanococcus thermolithotrophicus, Methanococcus deltae, and Methanobacterium thermoautotrophicum DeltaH and Marburg were completely inhibited by 7 muM 1,2-dibromoethane and, to various degrees, by 51 to 1,084 muM 1,2-dichloroethane, 1,2-dibromoethylene, 1,2-dichloroethylene, and trichloroethylene. In general, the brominated compounds were more inhibitory. The two Methanococcus species were fully inhibited by 1 muM bromoethanesulfonic acid, whereas both Methanobacterium strains were only partly inhibited by 2,124 muM. Coenzyme M protected cells from bromoethanesulfonic acid but not from any of the other inhibitors.