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. 1987 Jul;53(7):1705-7.
doi: 10.1128/aem.53.7.1705-1707.1987.

Identification of lotus rhizobia by direct DNA hybridization of crushed root nodules

J E Cooper  1 , A J Bjourson, J K Thompson
Affiliations

Identification of lotus rhizobia by direct DNA hybridization of crushed root nodules

J E Cooper et al. Appl Environ Microbiol. 1987 Jul.

Abstract

Hybridization of crushed Lotus pedunculatus root nodules with P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did not occur. Results were comparable to those from colony hybridization.

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References

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