Rhizobacteria of maize and their antifungal activities

Abstract

During the growing season of 1984, the rhizobacteria (including organisms from the rhizosphere soil, the rhizoplane, and internal root zones) of 47 maize plants (two varieties) sampled from different locations in France and at different growth stages were inventoried. Isolates were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of their total cell proteins and were found to represent 352 different protein electrotypes. Maize seedlings were initially colonized by a small number of different strains. Densities reached up to 10 CFU/g of root. Later in the season, the population density decreased but the heterogeneity of the rhizobacterial populations increased. Fluorescent pseudomonads represented up to 35% of the total rhizobacterial population and comprised 43 different electrotypes. Other bacteria regularly present were Xanthomonas maltophilia, Serratia liquefaciens, Pseudomonas paucimobilis, and Bacillus spp. There was a very low similarity between rhizobacterial populations of plants of the same cultivar (LG5) within one field at different growth stages and also between rhizobacterial populations of the cultivars LG5 and BRIO42 on the same field. Most electrotypes (76%) were found on a single occasion. None of the 352 electrotypes was present on all plants. In the 1985 analysis the rhizobacteria of maize seedlings (one variety) sampled from one field were characterized. They represented 236 different protein electrotypes. Thirty-three isolates showed antifungal activity against major maize pathogens; they comprised four Pseudomonas cepacia strains, producing pyrrolnitrin as well as another unknown antifungal compound.