Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Aug;53(8):1977-9.
doi: 10.1128/aem.53.8.1977-1979.1987.

Determining [H]Thymidine Incorporation into Bacterioplankton DNA: Improvement of the Method by DNase Treatment

P Servais  1 , J Martinez, G Billen, J Vives-Rego
Affiliations

Determining [H]Thymidine Incorporation into Bacterioplankton DNA: Improvement of the Method by DNase Treatment

P Servais et al. Appl Environ Microbiol. 1987 Aug.

Abstract

Determination of [H] thymidine incorporation into bacterial DNA versus other macromolecules is usually achieved by NaOH and hot trichloroacetic acid hydrolysis. This procedure was found not to be specific enough. An alternative method founded on DNase treatment is proposed. Under the new method, the fraction of thymidine incorporated into DNA ranged from 10 to 83%.

PubMed Disclaimer

References

    1. Methods Biochem Anal. 1966;14:113-76 - PubMed
    1. Appl Environ Microbiol. 1980 Jun;39(6):1085-95 - PubMed
    1. Appl Environ Microbiol. 1982 Jun;43(6):1393-9 - PubMed
    1. Appl Environ Microbiol. 1984 Dec;48(6):1221-30 - PubMed
    1. Schweiz Rundsch Med Prax. 1984 Jan 17;73(3):59-65 - PubMed

LinkOut - more resources