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. 1988 Feb;54(2):574-6.
doi: 10.1128/aem.54.2.574-576.1988.

A Positive Strain Identification Method for Rhizobium meliloti

R Wheatcroft  1 , R J Watson
Affiliations

A Positive Strain Identification Method for Rhizobium meliloti

R Wheatcroft et al. Appl Environ Microbiol. 1988 Feb.

Abstract

About 80% of Rhizobium meliloti strains contain 1 to 11 copies of insertion sequence ISRm1 in their genomes (R. Wheatcroft and R. J. Watson, J. Gen. Microbiol. 134:113-121, 1988). Hybridization to separated genomic DNA fragments with an ISRm1-specific probe produces patterns of hybridization bands which are distinctive for each strain. These patterns can be compared between strains to prove or disprove common identity. In most cases relatedness can be inferred despite phenotypic differences or minor genomic alterations.

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