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. 1988 Jun;54(6):1630-1.
doi: 10.1128/aem.54.6.1630-1631.1988.

A simple, rapid method for enumerating total viable and metabolically active bacteria in groundwater

L K King  1 , B C Parker
Affiliations

A simple, rapid method for enumerating total viable and metabolically active bacteria in groundwater

L K King et al. Appl Environ Microbiol. 1988 Jun.

Abstract

We report here a new staining procedure which uses both the enzymatic dehydrogenation of 2-(p-iodophenyl)-3-p-(nitrophenyl)-5 phenyltetrazolium chloride to a pink intracellular formazan and the DNA-specific fluorochrome 4',6'-diamidino-2-phenylindole. Application of this staining procedure to cells concentrated on filters and then transferred to microscope slides by the filter-transfer-freeze technique has proven valuable for statistically accurate enumeration of the total viable and metabolically active cells in groundwaters.

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References

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