doi: 10.1128/aem.54.12.3191-3193.1988.
Ethanol extraction requirement for purification of protein labeled with [h]leucine in aquatic bacterial production studies
Affiliations
- PMID: 16347811
- PMCID: PMC204452
- DOI: 10.1128/aem.54.12.3191-3193.1988
Item in Clipboard
Ethanol extraction requirement for purification of protein labeled with [h]leucine in aquatic bacterial production studies
Appl Environ Microbiol.
1988 Dec.
Abstract
The trichloroacetic acid (TCA)-insoluble fraction of water column bacteria labeled with [H]leucine contained an ethanol-soluble fraction accounting for up to 44% of the label. A component of the ethanol-soluble fraction is [H]leucine. Labeled-protein purification requires an ethanol wash step. Cold TCA can replace hot TCA for precipitation of labeled proteins.
Similar articles
-
Study of a novel agent for TCA precipitated proteins washing - comprehensive insights into the role of ethanol/HCl on molten globule state by multi-spectroscopic analyses.J Proteomics. 2018 Feb 20;173:77-88. doi: 10.1016/j.jprot.2017.11.016. Epub 2017 Nov 27. J Proteomics. 2018. PMID: 29191748
-
Comparison of ethanol plasma-protein precipitation with plasma ultrafiltration and trichloroacetic acid protein precipitation for the measurement of unbound platinum concentrations.Cancer Chemother Pharmacol. 1996;38(4):391-4. doi: 10.1007/s002800050501. Cancer Chemother Pharmacol. 1996. PMID: 8674165
-
Estimating bacterial production in marine waters from the simultaneous incorporation of thymidine and leucine.Appl Environ Microbiol. 1988 Aug;54(8):1934-9. doi: 10.1128/aem.54.8.1934-1939.1988. Appl Environ Microbiol. 1988. PMID: 16347706 Free PMC article.
-
[3H]Leucine incorporation method as a tool to measure secondary production by periphytic bacteria associated to the roots of floating aquatic macrophyte.J Microbiol Methods. 2007 Oct;71(1):23-31. doi: 10.1016/j.mimet.2007.06.020. Epub 2007 Jul 28. J Microbiol Methods. 2007. PMID: 17765986
-
Metabolism of 125I-labeled lipoproteins by the isolated rat lung.J Cell Biol. 1976 Jul;70(1):33-46. doi: 10.1083/jcb.70.1.33. J Cell Biol. 1976. PMID: 180034 Free PMC article.
Cited by
-
Impacts of tetracycline on planktonic bacterial production in prairie aquatic systems.Microb Ecol. 2007 Jul;54(1):52-5. doi: 10.1007/s00248-006-9170-5. Epub 2007 Jan 31. Microb Ecol. 2007. PMID: 17264995
-
Incorporation of [h]leucine and [h]valine into protein of freshwater bacteria: uptake kinetics and intracellular isotope dilution.Appl Environ Microbiol. 1992 Nov;58(11):3638-46. doi: 10.1128/aem.58.11.3638-3646.1992. Appl Environ Microbiol. 1992. PMID: 16348807 Free PMC article.
-
Bacterioplankton cell growth and macromolecular synthesis in seawater cultures during the North Atlantic Spring Phytoplankton Bloom, May, 1989.Microb Ecol. 1992 Sep;24(2):125-44. doi: 10.1007/BF00174450. Microb Ecol. 1992. PMID: 24193132
-
Bacterioplankton Production Determined by DNA Synthesis, Protein Synthesis, and Frequency of Dividing Cells in Tuamotu Atoll Lagoons and Surrounding Ocean.Microb Ecol. 1996 Jul;32(2):185-202. doi: 10.1007/BF00185887. Microb Ecol. 1996. PMID: 8688008
References
LinkOut - more resources
Full Text Sources
