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. 1989 Jan;55(1):266-7.
doi: 10.1128/aem.55.1.266-267.1989.

Cytokinins in Azotobacter vinelandii Culture Medium

B J Taller  1 , T Y Wong
Affiliations

Cytokinins in Azotobacter vinelandii Culture Medium

B J Taller et al. Appl Environ Microbiol. 1989 Jan.

Abstract

Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. The total cytokinin activity was equivalent to 0.75 mug of kinetin per liter.

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