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. 1989 May;55(5):1262-6.
doi: 10.1128/aem.55.5.1262-1266.1989.

Temperature Compensation in Methanosarcina barkeri by Modulation of Hydrogen and Acetate Affinity

P Westermann  1 , B K Ahring, R A Mah
Affiliations

Temperature Compensation in Methanosarcina barkeri by Modulation of Hydrogen and Acetate Affinity

P Westermann et al. Appl Environ Microbiol. 1989 May.

Abstract

The affinity of Methanosarcina barkeri 227 for acetate and hydrogen at different incubation temperatures was investigated. Increasing the temperature from 20 to 37 degrees C resulted in a 4.5-fold increase in K(m) for acetate and a 4.8-fold increase for hydrogen. The corresponding increase in V(max) for acetate was 8.3-fold (5.4-fold for hydrogen). This response implied a decrease in the temperature coefficient (Q(10)) and hence a decrease in the temperature dependency as a function of decreasing substrate concentration.

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