Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Jul;55(7):1818-22.
doi: 10.1128/aem.55.7.1818-1822.1989.

Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA

R Weller  1 , D M Ward
Affiliations

Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA

R Weller et al. Appl Environ Microbiol. 1989 Jul.

Abstract

Cloning of cDNA obtained from 16S rRNA (16S rcDNA) selectively retrieves species-specific sequence information useful for analyzing the composition and structure of natural microbial communities. With this technique we obtained recombinant 16S rcDNA libraries from Escherichia coli and from a model hot-spring cyanobacterial-mat community. The recombinant plasmids contained exclusively 16S rRNA-derived inserts. This selective approach is independent of biasing culture techniques and eliminates the laborious screening required to locate 16S rRNA gene-bearing recombinants in genomic DNA libraries obtained from natural communities.

PubMed Disclaimer

References

    1. J Bacteriol. 1988 Feb;170(2):720-6 - PubMed
    1. Appl Environ Microbiol. 1977 Oct;34(4):433-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5 - PubMed
    1. Syst Appl Microbiol. 1985;6:143-51 - PubMed
    1. Appl Environ Microbiol. 1988 May;54(5):1079-84 - PubMed

LinkOut - more resources