Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Aug;55(8):2025-2029.
doi: 10.1128/aem.55.8.2025-2029.1989.

Specific Dot-Immunobinding Assay for Detection and Enumeration of Thiobacillus ferrooxidans

Renato Arredondo  1 , Carlos A Jerez
Affiliations

Specific Dot-Immunobinding Assay for Detection and Enumeration of Thiobacillus ferrooxidans

Renato Arredondo et al. Appl Environ Microbiol. 1989 Aug.

Abstract

A specific and very sensitive dot-immunobinding assay for the detection and enumeration of the bioleaching microorganism Thiobacillus ferrooxidans was developed. Nitrocellulose spotted with samples was incubated with polyclonal antisera against whole T. ferrooxidans cells and then in I-labeled protein A or I-labeled goat antirabbit immunoglobulin G; incubation was followed by autoradiography. Since a minimum of 10 cells per dot could be detected, the method offers the possibility of simultaneous processing of numerous samples in a short time to monitor the levels of T. ferrooxidans in bioleaching operations.

PubMed Disclaimer

References

    1. Annu Rev Microbiol. 1986;40:311-36 - PubMed
    1. Arch Mikrobiol. 1973;88(4):285-98 - PubMed
    1. CRC Crit Rev Microbiol. 1978;6(3):207-6I - PubMed
    1. Annu Rev Microbiol. 1980;34:263-83 - PubMed
    1. Annu Rev Microbiol. 1984;38:265-92 - PubMed

LinkOut - more resources