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. 1989 Oct;55(10):2729-31.
doi: 10.1128/aem.55.10.2729-2731.1989.

Electron Spin Resonance Analysis of the Effect of Butanol on the Membrane Fluidity of Intact Cells of Clostridium acetobutylicum

S H Baer  1 , D L Bryant, H P Blaschek
Affiliations

Electron Spin Resonance Analysis of the Effect of Butanol on the Membrane Fluidity of Intact Cells of Clostridium acetobutylicum

S H Baer et al. Appl Environ Microbiol. 1989 Oct.

Abstract

Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical fluidizing effect of high (1.5%) butanol challenge by reducing its membrane fluidity at 12 and 30 h. The wild-type 824 strain was unable to so respond when challenged at the 1.5% level.

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