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. 1990 Jun;56(6):1967-70.
doi: 10.1128/aem.56.6.1967-1970.1990.

DNA Probe for Lactobacillus delbrueckii

M Delley  1 , B Mollet, H Hottinger
Affiliations

DNA Probe for Lactobacillus delbrueckii

M Delley et al. Appl Environ Microbiol. 1990 Jun.

Abstract

From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognizes L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an alpha-P-labeled DNA probe.

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