Effectiveness of Different Frankia Cell Types as Inocula for the Actinorhizal Plant Casuarina

Abstract

The soil bacterium Frankia of the Actinomycetales, capable of forming N(2)-fixing symbiotic root nodules on a diverse array of actinorhizal plants, has several morphological forms when grown in pure culture. Fresh hydrated preparations of whole cells, hyphae, and spores were all infective on seedlings of Casuarina at different dilutions. Desiccated hyphae showed no infection capacity, while desiccated spores remained infective, although at a reduced level. On the basis of most-probable-number statistics, spore suspensions were 3 orders of magnitude more infective than hyphae.