Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Aug;56(8):2584-2586.
doi: 10.1128/aem.56.8.2584-2586.1990.

Occurrence of an Affinity Site apart from the Active Site on the Raw-Starch-Digesting but Non-Raw-Starch-Adsorbable Bacillus subtilis 65 alpha-Amylase

Shinsaku Hayashida  1 , Yuji Teramoto, Takehiro Inoue, Shinji Mitsuiki
Affiliations

Occurrence of an Affinity Site apart from the Active Site on the Raw-Starch-Digesting but Non-Raw-Starch-Adsorbable Bacillus subtilis 65 alpha-Amylase

Shinsaku Hayashida et al. Appl Environ Microbiol. 1990 Aug.

Abstract

alpha-Cyclodextrin specifically inhibited raw starch digestion by Bacillus subtilis 65 alpha-amylase. The raw starch digestibility and alpha-cyclodextrin-Sepharose 6B adsorbability of this alpha-amylase were simultaneously lost when the specific domain corresponding to the affinity site essential for raw starch digestion was deleted by proteolysis. Occurrence of the affinity site on raw-starch-digesting enzymes was proven also with bacterial amylase.

PubMed Disclaimer

References

    1. Gene. 1987;51(1):43-52 - PubMed
    1. Appl Environ Microbiol. 1988 Jun;54(6):1516-22 - PubMed
    1. FEBS Lett. 1974 Oct 1;47(1):86-9 - PubMed
    1. Appl Environ Microbiol. 1986 Nov;52(5):1068-73 - PubMed
    1. Eur J Biochem. 1988 Jan 4;170(3):575-81 - PubMed