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. 1991 Mar;57(3):879-81.
doi: 10.1128/aem.57.3.879-881.1991.

Analysis of free amino acids in microbially colonized sandstone by precolumn phenyl isothiocyanate derivatization and high-performance liquid chromatography

J Siebert  1 , R J Palmer, P Hirsch
Affiliations

Analysis of free amino acids in microbially colonized sandstone by precolumn phenyl isothiocyanate derivatization and high-performance liquid chromatography

J Siebert et al. Appl Environ Microbiol. 1991 Mar.

Abstract

A fast, sensitive method for extraction and analysis of soluble free amino acids from microbially colonized sandstone is described. After precolumn phenyl isothiocyanate (PITC) derivatization, the PITC-amino acids were identified and quantified by reversed-phase high-performance liquid chromatography. This kind of analysis could be used to elucidate the role and function of amino acids in the nutrition of epi- and endolithic microorganisms active in biological weathering processes.

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