Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Oct;59(10):3498-501.
doi: 10.1128/aem.59.10.3498-3501.1993.

Induction and Regeneration of Autoplasts from Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200

R Peteranderl  1 , F Canganella, A Holzenburg, J Wiegel
Affiliations

Induction and Regeneration of Autoplasts from Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200

R Peteranderl et al. Appl Environ Microbiol. 1993 Oct.

Abstract

Autolysis was induced to form stable, cell wall-free cells of Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200, using a complex medium containing glycine (0.4% wt/vol) and/or sucrose or glycerol (10% wt/vol) at an optimum temperature of 64 degrees C. Autoplasts of both bacteria were grown as L-phase colonies on solid medium; more than 50% of these colonies regenerated to the walled form during prolonged incubation. The removal of the cell wall was confirmed by electron microscopy.

PubMed Disclaimer

References

    1. J Bacteriol. 1979 Sep;139(3):800-10 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2147-50 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2151-5 - PubMed
    1. J Bacteriol. 1976 May;126(2):869-82 - PubMed
    1. Appl Environ Microbiol. 1985 Oct;50(4):1097-9 - PubMed

LinkOut - more resources