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. 1962 Sep;10(5):401-6.
doi: 10.1128/am.10.5.401-406.1962.

Formation of Extracellular Sphingolipids by Microorganisms: IV. Pilot-Plant Production of Tetraacetylphytosphingosine by Hansenula ciferrii

H G Maister  1 , S P Rogovin, F H Stodola, L J Wickerham
Affiliations

Formation of Extracellular Sphingolipids by Microorganisms: IV. Pilot-Plant Production of Tetraacetylphytosphingosine by Hansenula ciferrii

H G Maister et al. Appl Microbiol. 1962 Sep.

Abstract

Tetraacetylphytosphingosine (TAPS) formation by the F-60-10 mating type strain of the yeast Hansenula ciferrii, previously observed on agar plates, has been shown to take place in submerged cultures. The optimal conditions for TAPS formation, and the correlation of TAPS production and sugar utilization under aerobic conditions, were studied in 10-liter fermentors. For each gram of glucose consumed, 5 mg of TAPS were formed; for each gram of yeast solids produced, 15 mg of TAPS were synthesized. A 750-liter pilot-plant run yielded 175 g of crude TAPS, which were obtained by hexane extraction of centrifuged yeast cells.

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References

    1. Appl Microbiol. 1957 Sep;5(5):313-8 - PubMed
    1. J Bacteriol. 1960 Oct;80:484-91 - PubMed

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