Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 Oct;24(4):551-4.
doi: 10.1128/am.24.4.551-554.1972.

Death of Lactobacillus bulgaricus Resulting from Liquid Nitrogen Freezing

R B Smittle  1 , S E Gilliland, M L Speck
Affiliations

Death of Lactobacillus bulgaricus Resulting from Liquid Nitrogen Freezing

R B Smittle et al. Appl Microbiol. 1972 Oct.

Abstract

Concentrated cell suspensions of Lactobacillus bulgaricus prepared from cells grown in semisynthetic media were frozen in liquid nitrogen. After storage for 24 hr, the cell suspensions were found to have decreased colony counts and acid-producing capacity in milk. The amount of loss varied among the different strains tested. The addition of known cryoprotective agents to cell suspensions of the most labile strain before freezing provided little or no protection to the cells. However, storage stability of all strains investigated was improved by supplementing the growth medium with Tween 80 (polyoxyethylene sorbitan monooleate). The concentration of Tween 80 necessary for maximal storage stability varied among strains.

PubMed Disclaimer

References

    1. Appl Microbiol. 1970 Jun;19(6):890-3 - PubMed
    1. Cryobiology. 1970 Nov-Dec;7(4):238-42 - PubMed
    1. Appl Microbiol. 1969 Jun;17(6):805-10 - PubMed
    1. Appl Microbiol. 1966 Jul;14(4):665-9 - PubMed
    1. J Bacteriol. 1951 Jul;62(1):132-3 - PubMed

LinkOut - more resources