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. 1992 Jul-Aug;29(7-8):1025-8.
doi: 10.1016/0161-5890(92)90142-k.

Sequences of variable regions of a monoclonal antibody specific to the thyroid hormone, triiodo-L-thyronine

P Thammana  1 , A L Gaito, M T Largen
Affiliations

Sequences of variable regions of a monoclonal antibody specific to the thyroid hormone, triiodo-L-thyronine

P Thammana et al. Mol Immunol. 1992 Jul-Aug.

Abstract

We have determined the nucleotide sequences of the variable regions of H and L chains of a monoclonal antibody 98QQ that interacts with the thyroid hormone triiodo-L- thyronine with high affinity. Analysis of the nucleotide sequence of the light chain V region of 98QQ revealed that the VL sequence is 99% identical to Balb/c germline Vk 21-E sequence. That is an interesting finding with this high affinity anti-T3 antibody, since occurence of predominantly germline variable region sequences is observed in some autoantibodies to self antigens but not usually in high affinity IgG antibodies. The sequence analysis also revealed that the heavy chain variable region sequence of 98QQ is similar to a V region of an anti-DNA antibody (MRL DNA 22). Thus the sequence analysis of our anti-T3 mAb 98QQ has revealed some features of autoantibodies to self antigens.

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