Review
doi: 10.1016/j.chembiol.2005.11.006.
The laboratory in a droplet
Affiliations
- PMID: 16356841
- DOI: 10.1016/j.chembiol.2005.11.006
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Review
The laboratory in a droplet
Chem Biol.
2005 Dec.
Abstract
In this issue of Chemistry and Biology, the groups of Tawfik and Griffiths present fluorescence-activated cell sorting of double emulsions as a generally applicable screen for enzyme activity. This novel methodology increases the throughput of a typical enzyme screen by two orders of magnitude.
Comment on
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High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments.Chem Biol. 2005 Dec;12(12):1281-9. doi: 10.1016/j.chembiol.2005.09.012. Chem Biol. 2005. PMID: 16356845
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High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions.Chem Biol. 2005 Dec;12(12):1291-300. doi: 10.1016/j.chembiol.2005.09.016. Chem Biol. 2005. PMID: 16356846
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