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. 2005 Dec;6(12):1148-52.
doi: 10.1631/jzus.2005.B1148.

Determination of theophylline concentration in serum by chemiluminescent immunoassay

Mei-xia Zhou  1 , Cha-ying Guan, Guang Chen, Xin-you Xie, Sheng-hai Wu
Affiliations

Determination of theophylline concentration in serum by chemiluminescent immunoassay

Mei-xia Zhou et al. J Zhejiang Univ Sci B. 2005 Dec.

Abstract

Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum.

Methods: To measure the concentration of theophylline (n=122) and evaluate the assay.

Results: The linear range of the CLIA method was 0.51-40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 micromol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L).

Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.

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Figures

Fig. 1
Fig. 1
The standard curve of theophylline
Fig. 2
Fig. 2
Correlation of CLIA method with FPIA method
See this image and copyright information in PMC

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