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. 2006 Jan;3(1):27-9.
doi: 10.1038/nmeth843.

In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes

Wigard P Kloosterman  1 , Erno Wienholds, Ewart de Bruijn, Sakari Kauppinen, Ronald H A Plasterk
Affiliations

In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes

Wigard P Kloosterman et al. Nat Methods. 2006 Jan.

Abstract

MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.

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Comment in

  • microRNA detection comes of age.
    Hammond SM. Hammond SM. Nat Methods. 2006 Jan;3(1):12-3. doi: 10.1038/nmeth0106-12. Nat Methods. 2006. PMID: 16369545 No abstract available.

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